Histotechnologist Practice Test

If you need help studying for the ASCP Histotechnologist exam or just want some more information about what the exam is like, you’ve come to the right place.

Click below to take a free Histotechnologist practice test!

Exam Eligibility

Before you can register to take the exam, you’ll have to meet the requirements in at least one of the eligibility routes below:

  1. You need a bachelor’s degree with at least 30 semester hours in biology and chemistry, and you need to have completed a histotechnician/histotechnologist program within the last five years.
  2. You need a bachelor’s degree with at least 30 semester hours in biology and chemistry, and you need at least one year of full-time histopathology lab experience or one year of full-time veterinary, industry, or research experience within the last five years.
  3. You need a valid HT(ASCP) certification, a bachelor’s degree, and at least six months of full-time histopathology lab experience within the last five years.
  4. You need a bachelor’s degree with at least 30 semester hours in biology and chemistry, and you need to have completed a 50-week military histotechnician training course within the last 10 years.

If you meet one of these requirements, you’re set to begin the registration process!

What’s on the Exam?

Questions: 100
Time limit: 2.5 hours

 
There are 100 multiple-choice questions total and the time limit for the exam is 2.5 hours. There aren’t any scheduled breaks, but you’re free to take restroom breaks as needed.

The exam itself is split into five sections, but the questions on the exam are further grouped into one of eight topics:

  • Tissues
  • Procedures
  • Parameters
  • Reagents
  • Instrumentation
  • Mounting procedures
  • Safety
  • Lab mathematics

Let’s take a closer look at the different sections of the Histotechnologist exam.


1. FIXATION
15%-25% of the exam

This section covers the following procedures:

  • Light microscopy
  • Artifacts/precipitates/pigments
  • Quality control
  • Cytologic specimens
  • In situ hybridization
  • Frozen sections/tissues
  • Enzyme histochemistry
  • Immunofluorescence
  • Electron microscopy
  • Special stains
  • Immunohistochemistry

2. PROCESSING
10%-20% of the exam

This section covers the following procedures:

  • Light microscopy
  • Quality control
  • Cytologic specimens
  • In situ hybridization
  • Frozen sections/tissues
  • Calcified/decalcified tissue
  • Enzyme histochemistry
  • Immunofluorescence
  • Immunohistochemistry

3. EMBEDDING/MICROTOMY
15%-25% of the exam

This section covers the following procedures:

  • Paraffin
  • Quality control
  • Frozen section
  • Preparation for molecular testing
  • Gelatin and adhesive

4. STAINING
30%-40% of the exam

This section covers the following procedures:

  • Carbohydrates
  • Connective/supporting tissue
  • Preliminary screening of histochemical special stains
  • Pigments, minerals, and granules
  • Immunohistochemistry
  • Tissues and cells, as well as their components
  • Quality control
  • Nucleus/cytoplasm
  • Bone marrow
  • Cytological stains
  • Enzyme histochemistry
  • Lipids
  • Microorganisms
  • Nerve

5. LABORATORY OPERATIONS
10%-15% of the exam

This section doesn’t contain any procedure-related questions. The main focus of these questions will be testing your laboratory mathematics knowledge, including the following:

  • Molar solutions
  • Percent solutions/dilutions
  • The metric system

Stains Covered on the Exam

Here’s a list of most of the stains you’ll encounter on the exam:

  • Alcian blue
  • Alcian blue-PAS
  • Alcian yellow
  • Bielschowsky
  • Carbol-fuchsin
  • Colloidal iron
  • Congo red
  • Crystal violet
  • Enzyme histochemistry
  • Fontana-Masson
  • Giemsa
  • Gram
  • Grocott/Gomori methenamine-silver
  • Melanin bleach
  • Hematoxylin and eosin
  • Immunohistochemical stains
  • Immunofluorescence
  • In situ hybridization
  • Luxol fast blue
  • Movat pentachrome
  • Mucicarmine
  • Oil red O
  • Papanicolaou
  • PAS-digestion
  • PAS-hematoxylin
  • Periodic acid-methenamine silver/Jones
  • Periodic acid-Schiff
  • Prussian blue
  • Reticulin/reticulum
  • Rhodanine
  • Romanowsky
  • Spirochete
  • Toluidine blue
  • Trichrome
  • Verhoeff-van Gieson
  • von Kossa

Computer-Adaptive Testing (CAT)

The Histotechnologist exam is a computer-adaptive test. Basically, this means that the questions will become harder or easier as you go through the exam, based on how well you’re answering the questions.

For example, say you answered the first question correctly. The first question is of medium difficulty, so the next question will be slightly harder. Then, let’s say you answered the second question incorrectly. The next question would then be a medium question.

How to Register

Once you’ve ensured that you meet all of the eligibility requirements, you can register for the exam!

To get started, you’ll need to submit an application on ASCP’s website. The application will ask you for your contact information and any documentation to prove your eligibility (among other things).

When you submit the application, you’ll also need to submit the $250 application fee.

Once your application is approved, you should receive an Authorization to Test (ATT), which will allow you to set up a testing appointment.

Histotechnologist Study Guide and Flashcards

Get practice questions, detailed study lessons, and complex subjects broken down into easily understandable concepts.

Study Guide

Exam Scores

The Histotechnologist test is scored using a scaled scoring method. Here’s how it works:

For every question you answer correctly, you get one point added to your raw score. At the end of the test, your final raw score will be converted to a scaled score. This scaled score will range somewhere between 100 and 999.

You will need a score of 400 to pass the test.

The reason your raw score is converted to a scaled score is because everyone who takes the test is given a slightly different set of questions. Since everyone has a different arrangement of questions, and because some questions are harder than others, converting your raw score to a scaled score ensures a more even playing field.

If you pass, you’ll get a certificate mailed to you within a couple of weeks!

Retaking the Exam

If you didn’t get a passing score on your first try, that’s okay! You can retake the test after a 90-day waiting period. Just keep in mind that you will have to pay the full testing fee every time you retake the test.

If you take the exam five times without passing, you won’t be able to take it again unless you are eligible under a different eligibility route.

FAQs

Q

How many questions are on the Histotechnologist exam?

A

The exam contains 100 questions.

Q

What is the time limit for the Histotechnologist exam?

A

The exam is timed at 2.5 hours.

Q

What is the passing score for the Histotechnologist exam?

A

You’ll need to get a final score of at least 400 to pass.

Q

How much does the Histotechnologist exam cost?

A

The testing fee is $250.