Medical Laboratory Technician Practice Test

If you need help studying for the Medical Laboratory Technician (MLT) exam or just want some more information about what the exam is like, you’ve come to the right place.

Click below to take a free MLT practice test!

Exam Eligibility

Before you can register to take the MLT exam, you’ll have to meet the requirements in at least one of the eligibility routes below:

  1. Education: You need an associate degree in medical laboratory technology from an accredited program/institution.
  2. Alternate Education: You need to have at least 60 semester hours, including 25 clinical laboratory science, math, and/or computer science hours. You also need at least 6 months of clinical laboratory experience.
  3. Military: You need to have finished a 50-week military medical laboratory training program.
  4. Other Exam: You need to have passed a generalist MLT exam from another certification bode or licensing agency.

If you meet one of these requirements, you’re set to begin the registration process!

What’s on the MLT Exam?

Questions: 100
Time limit: 2.5 hours

 
There are 100 multiple-choice questions total and the time limit for the exam is 2.5 hours. There aren’t any scheduled breaks, but you’re free to take restroom breaks as needed.

Let’s take a closer look at the different sections of the MLT exam.


1. BLOOD BANKING
15%-20% of the exam

  • Blood products
  • Blood group systems
  • Blood group immunology
  • Physiology
  • Pathophysiology
  • Serologic and molecular testing
  • Transfusion practice

2. URINALYSIS AND OTHER BODY FLUIDS
5%-10% of the exam

  • Physical and chemical testing
  • Microscopic analysis
  • Physiology
  • Disease states

3. CHEMISTRY
20%-25% of the exam

  • Carbohydrates
  • Lipids
  • Heme derivatives
  • Enzymes
  • Proteins
  • Acid-base determinations
  • Electrolytes
  • Endocrinology
  • Vitamins and nutrition
  • Therapeutic drug monitoring
  • Toxicology

4. HEMATOLOGY
20%-25% of the exam

  • Physiology
  • Disease states
  • Laboratory testing
  • Hemostasis

5. IMMUNOLOGY
5%-10% of the exam

  • Immunology principles
  • Diseases of the immune system
  • Transplantation
  • Infectious disease serology
  • Serologic procedures
  • Test results

6. MICROBIOLOGY
15%-20% of the exam

  • Preanalytic procedures
  • Analytic procedures for bacteriology
  • Analytic procedures for myobacteriology
  • Analytic procedures for virology
  • Analytic procedures for parasitology
  • Analytic procedures for mycology
  • Postanalytic procedures

7. LABORATORY OPERATIONS
5%-10% of the exam

  • Quality assessment and troubleshooting
  • Safety
  • Laboratory mathematics
  • Manual and automated methodology
  • Instrumentation

How to Register

Once you’ve ensured that you meet all of the eligibility requirements, you can register for the exam!

To get started, you’ll need to submit an application on ASCP’s website. The application will ask you for your contact information and any documentation to prove your eligibility (among other things).

When you submit the application, you’ll also need to submit the $225 application fee.

Once your application is approved, you should receive an Authorization to Test (ATT), which will allow you to set up a testing appointment.

MLT Study Guide and Flashcards

Get practice questions, detailed study lessons, and complex subjects broken down into easily understandable concepts.

Study Guide

Exam Scores

The MLT test is scored using a scaled scoring method. Here’s how it works:

For every question you answer correctly, you get one point added to your raw score. At the end of the test, your final raw score will be converted to a scaled score. This scaled score will range somewhere between 100 and 999.

You will need a score of 400 to pass the test.

The reason your raw score is converted to a scaled score is because everyone who takes the test is given a slightly different set of questions. Since everyone has a different arrangement of questions, and because some questions are harder than others, converting your raw score to a scaled score ensures a more even playing field.

If you pass, you’ll get a certificate mailed to you within a couple of weeks!

Retaking the Exam

If you didn’t get a passing score on your first try, that’s okay! You can retake the test after a 90-day waiting period. Just keep in mind that you will have to pay the full testing fee every time you retake the test.

If you take the exam five times without passing, you won’t be able to take it again unless you are eligible under a different eligibility route.

FAQs

Q

How many questions are on the MLT exam?

A

The exam contains 100 questions.

Q

What is the time limit for the MLT exam?

A

The exam is timed at 2.5 hours.

Q

What is the passing score for the MLT exam?

A

You’ll need to get a final scaled score of at least 400 to pass.

Q

How much does the MLT exam cost?

A

The application fee is $225.